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pegfp c1 vector backbone  (TaKaRa)


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    Structured Review

    TaKaRa pegfp c1 vector backbone
    Pegfp C1 Vector Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+vector+backbone/pmCherry-C1+Vector/pmc09759328-204-7-10
    Average 96 stars, based on 3289 article reviews
    pegfp c1 vector backbone - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Modification:

    Article Title: Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP-C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Article Title: Cancer associated talin point mutations disorganise cell adhesion and migration
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP–C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Article Title: Cancer associated talin point mutations disorganise cell adhesion and migration
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP-C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Plasmid Preparation:

    Article Title: Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP-C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Article Title: Cancer associated talin point mutations disorganise cell adhesion and migration
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP–C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: DOI: https://doi.org/10.7554/eLife.53670 17 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm FIJI (ImageJ) open access software, see https://imagej.net /Fiji/Downloads image processing Software, algorithm in-house Matlab codes open access, see Source code 1 image processing Other sticky-Slide VI 0.4 Ibidi 80608 flow chambers Other glass coverslips Ibidi 10812 functionalized substrates .. Human CaMKII-a (Uniprot_ID: Q9UQM7) and human CaMKII-b (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Cancer associated talin point mutations disorganise cell adhesion and migration
    Article Snippet: .. Talin variants were subcloned into a modified pEGFP-C1 vector backbone (Clontech). .. Cells were transfected with 6 μg plasmid DNA per 10 6 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V, 30 ms, one pulse.

    Variant Assay:

    Article Title: Phylogenetic convergence of phase separation and mitotic function in the disordered protein BuGZ.
    Article Snippet: Department of Biology, Johns Hopkins University, Baltimore, Maryland, USA Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland, USA T.C.. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA Translational Tissue Engineering Center, Department of Biomedical Engineering and Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Construct:

    Article Title: Phylogenetic convergence of phase separation and mitotic function in the disordered protein BuGZ.
    Article Snippet: Department of Biology, Johns Hopkins University, Baltimore, Maryland, USA Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland, USA T.C.. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA Translational Tissue Engineering Center, Department of Biomedical Engineering and Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Expressing:

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Transfection:

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Subcloning:

    Article Title: Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
    Article Snippet: .. Expression constructs and cell transfection EGFP- or mCherry-tagged talin-1 and vinculin expression constructs were created by subcloning of cDNA fragments encoding mouse talin-1, vinculin or EGFP/mCherry fluorescent proteins into a modified pEGFP-C1 vector backbone (Clontech). ..

    Clone Assay:

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: DOI: https://doi.org/10.7554/eLife.53670 17 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm FIJI (ImageJ) open access software, see https://imagej.net /Fiji/Downloads image processing Software, algorithm in-house Matlab codes open access, see Source code 1 image processing Other sticky-Slide VI 0.4 Ibidi 80608 flow chambers Other glass coverslips Ibidi 10812 functionalized substrates .. Human CaMKII-a (Uniprot_ID: Q9UQM7) and human CaMKII-b (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Sequencing:

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: .. Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..

    Article Title: Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation
    Article Snippet: DOI: https://doi.org/10.7554/eLife.53670 17 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm FIJI (ImageJ) open access software, see https://imagej.net /Fiji/Downloads image processing Software, algorithm in-house Matlab codes open access, see Source code 1 image processing Other sticky-Slide VI 0.4 Ibidi 80608 flow chambers Other glass coverslips Ibidi 10812 functionalized substrates .. Human CaMKII-a (Uniprot_ID: Q9UQM7) and human CaMKII-b (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag, GLNDIFEAQKIEWHE) followed by a linker (GASGASGASGAS) at the N-terminus of mEGFP. ..



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